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KMID : 0384119930130040569
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 4 p.569 ~ p.578
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Expression of p53 Protein in Human Leukemia Cells
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Abstract
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p53 has characteristics of a tumor-suppressor gene whose loss-of-function mutations are oncogenic. Mutant p53 protein is more stable with a longer half life, and this stability allows it to be detected using immunocytochemical stain, whereas the
short
half-life of wild type p53 makes this difficult. We established labelled streptavidin-biotin technique with anti-p53 monoclonal antibodies, PAb1801 and PAb240 for detection of p53 protein and used it to study the expression of p53 in 11 leukemic
cell
lines and 55 cases of human leukemias. In comparison of fixation methods, the cells fixed in 95% acetone for 5 minutes and 0.1% Triton X-100 for 5 minutes at room temperature showed excellent nuclear positivity. The expression of p53 was
demonstrated in
nine cell lines and 10 acute leukemias (seven common ALL, two AML, one T-ALL). p53 protein was also detected in one out of five cases of leukemic relapses. The positivities with the different monoclonal antibodies were variable and the expression
of p53
protein was not related to immunologic phenotypes of leukemia cells. To determine the clinical significance of p53 protein expression in acute leukemia, further clinical studies are needed.
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KEYWORD
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